Difference in the lethal effects of carcinogens/mutagens among three cultured goldfish (Carassius auratus) cell lines.

Fish/Radiosensitivity/Colony formation/Mutagens The sensitivities of three goldfish-cell lines to the lethal effects of four carcinogens/mutagens; 4-nitro quinoline-1-oxide (4NQO), 1-methyl-3-nitro-l-nitrosoguanidine (MNNG), mitomycin C (MMC) and bleo mycin (BLM), were compared using colony-formation assay. RBCF-1 and CAF-MM1 cells were more sensitive to 4NQO, BLM and UV than were GEM 199 cells. CAF-MM1 and GEM 199 cells showed almost he same sensitivity to MNNG, while the RBCF-1 cells were more sensitive. RBCF-1 cells were also the most sensitive to MMC and BLM among the three. This suggests that there may be some defect in DNA repair system in RBCF-1 cell ine.